{"id":159596,"date":"2010-01-01T00:00:00","date_gmt":"2010-01-01T00:00:00","guid":{"rendered":"https:\/\/www.microsoft.com\/en-us\/research\/msr-research-item\/disruption-of-two-defensive-signaling-pathways-by-a-viral-rna-silencing-suppressor\/"},"modified":"2018-10-16T21:30:20","modified_gmt":"2018-10-17T04:30:20","slug":"disruption-of-two-defensive-signaling-pathways-by-a-viral-rna-silencing-suppressor","status":"publish","type":"msr-research-item","link":"https:\/\/www.microsoft.com\/en-us\/research\/publication\/disruption-of-two-defensive-signaling-pathways-by-a-viral-rna-silencing-suppressor\/","title":{"rendered":"Disruption of two defensive signaling pathways by a viral RNA silencing suppressor"},"content":{"rendered":"<div class=\"asset-content\">\n<p>The Cucumber mosaic virus (CMV) 2b counter-defense protein disrupts plant antiviral mechanisms mediated by RNA silencing and salicylic acid (SA). We used microarrays to investigate\u00a0 defensive gene expression in 2b-transgenic Arabidopsis thaliana plants. Surprisingly, 2b inhibited expression of few SA-regulated genes and, in some instances, enhanced the effect of SA on certain genes. Strikingly, the 2b protein inhibited changes in the expression of 90% of genes regulated by jasmonic acid (JA). Consistent with this, infection of plants with CMV, but not the 2b\u00a0 gene-deletion mutant CMV\u03942b, strongly inhibited JA-inducible gene expression. JA levels were unaffected by infection with either CMV or CMV\u03942b. Although the CMV\u2013Arabidopsis interaction is a compatible one, SA accumulation, usually considered to be an indicator of plant resistance, was increased in CMV-infected plants but not in CMV\u03942b-infected plants. Thus, the 2b protein inhibits JA signaling at a step downstream of JA biosynthesis but it primes induction of SA biosynthesis by another CMV gene product or by the process of infection itself. Like many plant viruses, CMV is aphid transmitted. JA is important in plant defense against insects. This raises the possibility that disruption of JA-mediated gene expression by the 2b protein may influence CMV transmission by aphids.<\/p>\n<\/div>\n<p><!-- .asset-content --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Cucumber mosaic virus (CMV) 2b counter-defense protein disrupts plant antiviral mechanisms mediated by RNA silencing and salicylic acid (SA). We used microarrays to investigate\u00a0 defensive gene expression in 2b-transgenic Arabidopsis thaliana plants. Surprisingly, 2b inhibited expression of few SA-regulated genes and, in some instances, enhanced the effect of SA on certain genes. Strikingly, the [&hellip;]<\/p>\n","protected":false},"featured_media":0,"template":"","meta":{"msr-url-field":"","msr-podcast-episode":"","msrModifiedDate":"","msrModifiedDateEnabled":false,"ep_exclude_from_search":false,"_classifai_error":"","msr-author-ordering":null,"msr_publishername":"","msr_publisher_other":"","msr_booktitle":"","msr_chapter":"","msr_edition":"Molecular Plant-Microbe Interations","msr_editors":"","msr_how_published":"","msr_isbn":"","msr_issue":"","msr_journal":"Molecular Plant-Microbe Interations","msr_number":"7","msr_organization":"","msr_pages_string":"835-845","msr_page_range_start":"835","msr_page_range_end":"845","msr_series":"","msr_volume":"23","msr_copyright":"","msr_conference_name":"","msr_doi":"","msr_arxiv_id":"","msr_s2_paper_id":"","msr_mag_id":"","msr_pubmed_id":"","msr_other_authors":"Mathew G Lewsey, Alex M Murphy, Daniel MacLean, Jack H Westwood, Keith Macaulay, Mark H Bennett, Michael Moulin, David E Hanke, Glen Powell, Alison G Smith, John 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We continue to actively explore the exciting intersection of computing and life sciences, with other projects located on\u00a0www.microsoft.com\/research. Building a platform for programming biology The ability to program biology could enable fundamental breakthroughs across a broad range of industries, including medicine, agriculture, food, construction, textiles, materials and chemicals. It could also help lay the foundation for a future bioeconomy based on sustainable technology. 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