Stabilizing Synthetic DNA for Long-term Data Storage with Earth Alkaline Salts
- A. Xavier Kohll ,
- Philipp L. Antkowiak ,
- Weida D. Chen ,
- Bichlien Nguyen ,
- Wendelin J. Stark ,
- Luis Ceze ,
- Karin Strauss ,
- Robert N. Grass
Chemical Communications |
Rapid aging tests (70 °C, 50% RH) of solid state DNA dried in the presence of various salt formulations, showed the strong stabilizing effect of calcium phosphate, calcium chloride and magnesium chloride, even at high DNA loadings (>20 wt%). A DNA-based digital information storage system utilizing the stabilizing effect of MgCl2 was tested by storing a DNA file, encoding 115 kB of digital data, and the successful readout of the file by sequencing after accelerated aging.